CRL-2553 Panc 02.03 人胰腺癌細胞

ATCC® Number: CRL-2553™ Price: $417.00

Designations: Panc 02.03

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

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CRL-2553 Panc 02.03 人胰腺癌細胞

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

DNA Profile （STR）: Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 9,10

THO1: 6

TPOX: 9,12

vWA: 17

Age: 70 years

Gender: female

Ethnicity: White

Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 28.2 hrs [50655]

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

CRL-2553

來自ATCC細胞，所有出入庫細胞均經(jīng)過嚴格的細胞質(zhì)量檢測，確保細胞無污染，符合檢測合格標準。歡迎來詢價詳談